{"id":9794,"date":"2024-10-22T15:18:35","date_gmt":"2024-10-22T07:18:35","guid":{"rendered":"https:\/\/sklmo.tsinghua.edu.cn\/?p=9794"},"modified":"2024-10-22T15:18:37","modified_gmt":"2024-10-22T07:18:37","slug":"%e6%b8%85%e5%8d%8e%e5%a4%a7%e5%ad%a6%e8%93%9d%e5%8b%8b%e3%80%81%e6%9d%8e%e5%af%85%e9%9d%92%e3%80%81%e6%9c%b1%e6%98%8e%e5%9b%a2%e9%98%9f%e5%90%88%e4%bd%9c%e6%8e%a8%e5%87%ba%e9%80%9a%e7%94%a8%e5%9e%8b","status":"publish","type":"post","link":"https:\/\/sklmo.tsinghua.edu.cn\/?p=9794","title":{"rendered":"\u6e05\u534e\u5927\u5b66\u84dd\u52cb\u3001\u674e\u5bc5\u9752\u3001\u6731\u660e\u56e2\u961f\u5408\u4f5c\u63a8\u51fa\u901a\u7528\u578b\u57fa\u56e0\u7f16\u8f91\u8131\u9776\u68c0\u6d4b\u6280\u672f"},"content":{"rendered":"\n<p>\u5206\u5b50\u80bf\u7624\u5b66\u5168\u56fd\u91cd\u70b9\u5b9e\u9a8c\u5ba4\uff08\u6e05\u534e\uff09<strong>\u84dd\u52cb\u8bfe\u9898\u7ec4<\/strong>\u4e0e<strong>\u674e\u5bc5\u9752\u8bfe\u9898\u7ec4<\/strong>\u5408\u4f5c\u4e8e2024\u5e747\u67082\u65e5\u5728<em><strong>\u300aNature Biotechnology\u300b<\/strong><\/em>\u671f\u520a\u4e0a\u53d1\u8868\u4e86\u9898\u4e3a<em><strong>\u201c&nbsp;Tracking-seq reveals the heterogeneity of off-target effects in CRISPR\/Cas9-mediated genome editing&nbsp;\u201d&nbsp;<\/strong><\/em>\u7684\u7814\u7a76\u8bba\u6587\uff0c\u62a5\u9053\u4e86\u4e00\u9879\u540d\u4e3aTracking-seq\u7684\u65b0\u578b\u8131\u9776\u6548\u5e94\u68c0\u6d4b\u6280\u672f\uff0c\u5e76\u501f\u52a9\u8be5\u6280\u672f\u63ed\u793a\u4e86\u4e0d\u540c\u7ec6\u80de\u7c7b\u578b\u3001\u4e0d\u540c\u7f16\u8f91\u5de5\u5177\u95f4\u5b58\u5728\u7684\u8131\u9776\u6548\u5e94\u5f02\u8d28\u6027\u3002\u6b64\u9879\u7814\u7a76\u5c06\u4e3a\u57fa\u56e0\u7f16\u8f91\u5728\u57fa\u56e0\u6cbb\u7597\u7b49\u9886\u57df\u7684\u5b89\u5168\u5e94\u7528\u63d0\u4f9b\u91cd\u8981\u7406\u8bba\u53c2\u8003\u53ca\u6280\u672f\u652f\u6301\u3002<\/p>\n\n\n\n<p>\u8fd1\u5e74\u6765\uff0c\u968f\u7740\u8bf8\u5982\u80de\u5627\u5576\u78b1\u57fa\u7f16\u8f91\u5668\uff08cytosine base editor, 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protein A, RPA\uff09\u5e7f\u6cdb\u5b58\u5728\u4e8e\u57fa\u56e0\u7ec4\u53cc\u94fe\u53ca\u5355\u94fe\u635f\u4f24\u4fee\u590d\u8fc7\u7a0b\uff0cTracking-seq\u901a\u8fc7CUT&amp;RUN\u6280\u672f\u7279\u5f02\u6027\u5730\u8bc6\u522b\u5e76\u6355\u83b7\u4e0eRPA\u7ed3\u5408\u7684\u5355\u94feDNA\uff08Single-stranded DNA, ssDNA\uff09\uff0c\u968f\u540e\u5229\u7528\u94fe\u7279\u5f02\u6027\u6838\u9178\u9176\u6d88\u5316\u53cc\u94feDNA\u4ee5\u63d0\u9ad8\u4fe1\u566a\u6bd4\uff0c\u6784\u5efa\u94fe\u7279\u5f02\u6027ssDNA\u6587\u5e93\u8fdb\u884c\u9ad8\u901a\u91cf\u6d4b\u5e8f\uff0c\u5e76\u901a\u8fc7\u65b0\u5f00\u53d1\u7684\u7b97\u6cd5\u7cbe\u786e\u8ba1\u7b97RPA\u4fe1\u53f7\u5f3a\u5ea6\uff0c\u8fdb\u800c\u5b9e\u73b0\u5bf9\u591a\u79cd\u7f16\u8f91\u5de5\u5177\u7684\u8131\u9776\u68c0\u6d4b\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img fetchpriority=\"high\" decoding=\"async\" width=\"830\" height=\"206\" src=\"https:\/\/sklmo.tsinghua.edu.cn\/wp-content\/uploads\/2024\/10\/image-9.png\" alt=\"\" class=\"wp-image-9795\" srcset=\"https:\/\/sklmo.tsinghua.edu.cn\/wp-content\/uploads\/2024\/10\/image-9.png 830w, https:\/\/sklmo.tsinghua.edu.cn\/wp-content\/uploads\/2024\/10\/image-9-300x74.png 300w, https:\/\/sklmo.tsinghua.edu.cn\/wp-content\/uploads\/2024\/10\/image-9-768x191.png 768w\" sizes=\"(max-width: 830px) 100vw, 830px\" \/><figcaption class=\"wp-element-caption\">\u56fe. Tracking-seq\u793a\u610f\u56fe<\/figcaption><\/figure>\n\n\n\n<p>\u76f8\u8f83\u4e8e\u73b0\u6709\u7684GUIDE-seq\u548cDISCOVER-seq\u7b49\u8131\u9776\u68c0\u6d4b\u6280\u672f\uff0cTracking-seq\u4e0d\u4ec5\u5c55\u73b0\u4e86\u9ad8\u53ec\u56de\u7387\uff0c\u800c\u4e14\u5177\u5907\u5f88\u9ad8\u7684\u68c0\u6d4b\u7cbe\u786e\u5ea6\u3002\u91cd\u8981\u7684\u662f\uff0c\u5176\u5177\u6709\u9ad8\u5ea6\u7684\u6cdb\u7528\u6027\uff0c\u4f7f\u5176\u80fd\u591f\u9002\u7528\u4e8eCas9\u3001CBE\u3001ABE\u4ee5\u53caPE\u7b49\u5404\u7c7b\u4e3b\u6d41\u57fa\u56e0\u7f16\u8f91\u5de5\u5177\uff0c\u5e76\u80fd\u9002\u5e94\u4e0d\u540c\u7684\u7ec6\u80de\u7c7b\u578b\u53ca\u4e0d\u540c\u7684\u7f16\u8f91\u73af\u5883\uff08\u5305\u62ec\u4f53\u5916\u3001\u79bb\u4f53\u53ca\u4f53\u5185\u7f16\u8f91\uff09\u3002\u6b64\u5916\uff0cTracking-seq\u7684\u9ad8\u7075\u654f\u5ea6\u4f7f\u5176\u80fd\u591f\u5728\u4ec5\u6709\u6570\u5343\u4e2a\u7ec6\u80de\u7684\u6837\u54c1\u4e2d\uff0c\u6709\u6548\u5730\u8fdb\u884c\u8131\u9776\u68c0\u6d4b\uff0c\u5e76\u4fdd\u6301\u4f18\u5f02\u7684\u7ed3\u679c\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"830\" height=\"284\" src=\"https:\/\/sklmo.tsinghua.edu.cn\/wp-content\/uploads\/2024\/10\/image-10.png\" alt=\"\" class=\"wp-image-9796\" srcset=\"https:\/\/sklmo.tsinghua.edu.cn\/wp-content\/uploads\/2024\/10\/image-10.png 830w, https:\/\/sklmo.tsinghua.edu.cn\/wp-content\/uploads\/2024\/10\/image-10-300x103.png 300w, https:\/\/sklmo.tsinghua.edu.cn\/wp-content\/uploads\/2024\/10\/image-10-768x263.png 768w\" sizes=\"(max-width: 830px) 100vw, 830px\" \/><figcaption class=\"wp-element-caption\">\u56fe.\u00a0Tracking-seq\u68c0\u6d4b\u4e0d\u540c\u7f16\u8f91\u5de5\u5177\u7684\u94fe\u7279\u5f02\u6027\u4fe1\u53f7<\/figcaption><\/figure>\n\n\n\n<p>\u5f97\u76ca\u4e8eTracking-seq\u7684\u9ad8\u6cdb\u7528\u6027\uff0c\u672c\u7814\u7a76\u53d1\u73b0\u5f53\u4f7f\u7528\u76f8\u540c\u7684\u5411\u5bfcRNA\u65f6\uff0cCas9\u3001CBE\u3001ABE\u548cPE\u7684\u8131\u9776\u6548\u5e94\u5b58\u5728\u660e\u663e\u5dee\u5f02\uff0c\u5176\u4e2dCas9\u548cCBE\u4e4b\u95f4\u89c2\u6d4b\u5230\u4e86\u8bb8\u591a\u8fd1\u4f3c\u4e8e\u5404\u81ea\u7279\u5f02\u7684\u8131\u9776\u4f4d\u70b9\u3002\u6b64\u5916\uff0c\u4e0d\u540c\u7c7b\u578b\u7684\u7ec6\u80de\u4e2d\u4e5f\u89c2\u5bdf\u5230\u4e86\u8131\u9776\u6548\u5e94\u7684\u5f02\u8d28\u6027\uff0c\u8131\u9776\u4e8b\u4ef6\u4f3c\u4e4e\u66f4\u5bb9\u6613\u5728\u67d3\u8272\u8d28\u5f00\u653e\u548c\u6d3b\u8dc3\u7684\u533a\u57df\u53d1\u751f\u3002\u8fd9\u4e9b\u8131\u9776\u6548\u5e94\u7684\u5f02\u8d28\u6027\u63d0\u793a\uff0c\u4f7f\u7528\u66ff\u4ee3\u6027\u7f16\u8f91\u7cfb\u7edf\u6216\u5728\u4e0d\u540c\u7ec6\u80de\u7c7b\u578b\u4e2d\u8fdb\u884c\u7684\u8131\u9776\u68c0\u6d4b\uff0c\u53ef\u80fd\u65e0\u6cd5\u51c6\u786e\u53cd\u6620\u76ee\u6807\u7ec6\u80de\u4e2d\u5b9e\u9645\u7684\u8131\u9776\u60c5\u51b5\u3002\u76f8\u6bd4\u4e4b\u4e0b\uff0cTracking-seq\u6280\u672f\u80fd\u591f\u76f4\u63a5\u5bf9\u539f\u59cb\u7f16\u8f91\u7cfb\u7edf\u8fdb\u884c\u8131\u9776\u68c0\u6d4b\uff0c\u56e0\u6b64\u5177\u6709\u5e7f\u9614\u7684\u5e94\u7528\u6f5c\u529b\u548c\u524d\u666f\u3002<\/p>\n\n\n\n<p>\u6e05\u534e\u5927\u5b66\u84dd\u52cb\u526f\u6559\u6388\u3001\u674e\u5bc5\u9752\u526f\u6559\u6388\u4ee5\u53ca\u535a\u58eb\u540e\u6731\u660e\u4e3a\u672c\u7814\u7a76\u7684\u5171\u540c\u901a\u8baf\u4f5c\u8005\u3002\u6e05\u534e\u5927\u5b66\u57fa\u7840\u533b\u5b66\u9662\u535a\u58eb\u540e\u6731\u660e\u535a\u58eb\u30012018\u7ea7\u535a\u58eb\u751f\u8bb8\u6da6\u8fbe\u3001\u836f\u5b66\u96622017\u7ea7\u535a\u58eb\u751f\u8881\u4fca\u677e\u548c\u751f\u547d\u5b66\u96622021\u7ea7\u535a\u58eb\u751f\u738b\u5609\u7a0b\u4e3a\u5171\u540c\u7b2c\u4e00\u4f5c\u8005\u3002\u6b64\u5916\uff0c\u8be5\u7814\u7a76\u8fd8\u5f97\u5230\u4e86\u6e05\u534e\u5927\u5b66\u675c\u5a1f\u5a1f\u7814\u7a76\u5458\u56e2\u961f\u3001\u6797\u6b23\u6559\u6388\u56e2\u961f\u3001\u9889\u4f1f\u6559\u6388\u56e2\u961f\uff0c\u70df\u53f0\u6bd3\u749c\u9876\u533b\u9662\u6797\u6625\u534e\u6559\u6388\u56e2\u961f\u3001\u6842\u6797\u533b\u5b66\u9662\u4e8e\u9e3f\u6d69\u6559\u6388\u56e2\u961f\u7684\u91cd\u8981\u5e2e\u52a9\u3002\u540c\u65f6\u611f\u8c22\u6e05\u534e\u5927\u5b66\u7941\u6d77\u56e2\u961f\u53ca\u4e2d\u56fd\u79d1\u5b66\u9662\u52a8\u7269\u7814\u7a76\u6240\u9b4f\u59a5\u7814\u7a76\u5458\u56e2\u961f\u7684\u5e2e\u52a9\u3002&nbsp;<\/p>\n\n\n\n<p>\u8be5\u9879\u76ee\u53d7\u5230\u56fd\u5bb6\u91cd\u70b9\u7814\u53d1\u8ba1\u5212\u3001\u56fd\u5bb6\u81ea\u7136\u79d1\u5b66\u57fa\u91d1\u3001\u5317\u4eac\u5e02\u81ea\u7136\u79d1\u5b66\u57fa\u91d1\u3001\u6e05\u534e\u5317\u5927\u751f\u547d\u8054\u5408\u4e2d\u5fc3\u3001\u6e05\u534e\u5927\u5b66\u81ea\u4e3b\u79d1\u7814\u8ba1\u5212\u7684\u8d44\u52a9\u3002<\/p>\n\n\n\n<p><strong>\u539f\u6587\u94fe\u63a5\uff1a<\/strong><\/p>\n\n\n\n<p><a href=\"https:\/\/www.nature.com\/articles\/s41587-024-02307-y\">https:\/\/www.nature.com\/articles\/s41587-024-02307-y<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5206\u5b50\u80bf\u7624\u5b66\u5168\u56fd\u91cd\u70b9\u5b9e\u9a8c\u5ba4\uff08\u6e05\u534e\uff09\u84dd\u52cb\u8bfe\u9898\u7ec4\u4e0e\u674e\u5bc5\u9752\u8bfe\u9898\u7ec4\u5408\u4f5c [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":9795,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[22],"tags":[],"class_list":["post-9794","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news"],"_links":{"self":[{"href":"https:\/\/sklmo.tsinghua.edu.cn\/index.php?rest_route=\/wp\/v2\/posts\/9794","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sklmo.tsinghua.edu.cn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sklmo.tsinghua.edu.cn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sklmo.tsinghua.edu.cn\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sklmo.tsinghua.edu.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9794"}],"version-history":[{"count":1,"href":"https:\/\/sklmo.tsinghua.edu.cn\/index.php?rest_route=\/wp\/v2\/posts\/9794\/revisions"}],"predecessor-version":[{"id":9797,"href":"https:\/\/sklmo.tsinghua.edu.cn\/index.php?rest_route=\/wp\/v2\/posts\/9794\/revisions\/9797"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sklmo.tsinghua.edu.cn\/index.php?rest_route=\/wp\/v2\/media\/9795"}],"wp:attachment":[{"href":"https:\/\/sklmo.tsinghua.edu.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9794"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sklmo.tsinghua.edu.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9794"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sklmo.tsinghua.edu.cn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9794"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}