{"id":9753,"date":"2024-10-22T13:54:28","date_gmt":"2024-10-22T05:54:28","guid":{"rendered":"https:\/\/sklmo.tsinghua.edu.cn\/?p=9753"},"modified":"2024-10-22T13:54:29","modified_gmt":"2024-10-22T05:54:29","slug":"%e5%88%86%e5%ad%90%e8%82%bf%e7%98%a4%e5%ad%a6%e5%85%a8%e5%9b%bd%e9%87%8d%e7%82%b9%e5%ae%9e%e9%aa%8c%e5%ae%a4%ef%bc%88%e6%b8%85%e5%8d%8e%ef%bc%897-9%e6%9c%88%e7%a7%91%e7%a0%94%e8%bf%9b%e5%b1%95","status":"publish","type":"post","link":"https:\/\/sklmo.tsinghua.edu.cn\/?p=9753","title":{"rendered":"\u5206\u5b50\u80bf\u7624\u5b66\u5168\u56fd\u91cd\u70b9\u5b9e\u9a8c\u5ba4\uff08\u6e05\u534e\uff097-9\u6708\u79d1\u7814\u8fdb\u5c55"},"content":{"rendered":"\n

7\u6708<\/strong><\/h2>\n\n\n\n

1.\u6e05\u534e\u5927\u5b66\u57fa\u7840\u533b\u5b66\u9662\u3001\u5206\u5b50\u80bf\u7624\u5b66\u5168\u56fd\u91cd\u70b9\u5b9e\u9a8c\u5ba4\u4e3b\u8981\u6210\u5458\u84dd\u52cb\u526f\u6559\u6388\u8bfe\u9898\u7ec4\u4e0e\u836f\u5b66\u9662\u3001\u5206\u5b50\u80bf\u7624\u5b66\u5168\u56fd\u91cd\u70b9\u5b9e\u9a8c\u5ba4\u4e3b\u8981\u6210\u5458\u674e\u5bc5\u9752\u526f\u6559\u6388\u8bfe\u9898\u7ec4\u5408\u4f5c\u4e8e2024\u5e747\u67082\u65e5\u5728\u300aNature Biotechnology\u300b\u671f\u520a\u4e0a\u53d1\u8868\u4e86\u9898\u4e3a”Tracking-seq reveals the heterogeneity of off-target effects in CRISPR\/Cas9-mediated genome editing”\u7684\u7814\u7a76\u8bba\u6587\uff0c\u62a5\u9053\u4e86\u4e00\u9879\u540d\u4e3aTracking-seq\u7684\u65b0\u578b\u8131\u9776\u6548\u5e94\u68c0\u6d4b\u6280\u672f\uff0c\u5e76\u501f\u52a9\u8be5\u6280\u672f\u63ed\u793a\u4e86\u4e0d\u540c\u7ec6\u80de\u7c7b\u578b\u3001\u4e0d\u540c\u7f16\u8f91\u5de5\u5177\u95f4\u5b58\u5728\u7684\u8131\u9776\u6548\u5e94\u5f02\u8d28\u6027\u3002\u6b64\u9879\u7814\u7a76\u5c06\u4e3a\u57fa\u56e0\u7f16\u8f91\u5728\u57fa\u56e0\u6cbb\u7597\u7b49\u9886\u57df\u7684\u5b89\u5168\u5e94\u7528\u63d0\u4f9b\u91cd\u8981\u7406\u8bba\u53c2\u8003\u53ca\u6280\u672f\u652f\u6301\u3002<\/p>\n\n\n\n

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Tracking-seq\u793a\u610f\u56fe<\/figcaption><\/figure>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Tracking-seq\u68c0\u6d4b\u4e0d\u540c\u7f16\u8f91\u5de5\u5177\u7684\u94fe\u7279\u5f02\u6027\u4fe1\u53f7<\/p>\n\n\n\n

\u539f\u6587\u94fe\u63a5\uff1ahttps:\/\/www.nature.com\/articles\/s41587-024-02307-y<\/a><\/p>\n\n\n\n

2.\u6e05\u534e\u5927\u5b66\u836f\u5b66\u9662\u3001\u5206\u5b50\u80bf\u7624\u5b66\u5168\u56fd\u91cd\u70b9\u5b9e\u9a8c\u5ba4\u4e3b\u8981\u6210\u5458\u9976\u71cf\u6559\u6388\u8bfe\u9898\u7ec4\u4e0e\u6606\u660e\u533b\u79d1\u5927\u5b66\u751f\u7269\u533b\u5b66\u5de5\u7a0b\u5b66\u9662\u9648\u7b56\u5b9e\u9662\u957f\u56e2\u961f\u5408\u4f5c\u4e8e2024\u5e747\u67089\u65e5\u5728\u300aCancer Letters\u300b\u5728\u7ebf\u53d1\u8868\u4e86\u9898\u4e3a” PI3K PROTAC overcomes the lapatinib resistance in PIK3CA-mutant HER2 positive breast cancer “\u7684\u8bba\u6587\uff0c\u8be5\u7814\u7a76\u663e\u793a\uff0cPI3K PROTAC\u5206\u5b50\u5bf9\u4e8e\u643a\u5e26PIK3CA\u57fa\u56e0\u7a81\u53d8\u7684\u4e73\u817a\u764c\u7ec6\u80de\u7cfb\u5177\u6709\u66f4\u654f\u611f\u7684\u9009\u62e9\u6027\u6740\u4f24\u4f5c\u7528\u3002\u5728HER2+\u7684\u5929\u7136\u8010\u66f2\u59a5\u73e0\u5355\u6297\u53ca\u62c9\u5e15\u66ff\u5c3c\u7684\u4e24\u682a\u7ec6\u80de\u7cfbJIMT1\u53caMDA-MB-453\u4e2d\uff0cPI3K PROTAC\u5206\u5b50\u5448\u73b0\u4e86\u6781\u4f4e\u7684IC50\uff0875 nM\uff0c87 nM\uff09\u3001DC50\uff086 nM, 2 nM\uff09\u53caT1\/2\uff082.5 nM\/11 h\uff0c2 nM\/8.1 h\uff09\u3002\u8fdb\u4e00\u6b65\u7684\u7814\u7a76\u53d1\u73b0\uff0c\u6781\u4f4e\u6d53\u5ea6\u7684PI3K PROTAC\u5206\u5b50\u53ef\u534f\u540c\u62c9\u5e15\u66ff\u5c3c\uff0c\u589e\u52a0\u8010\u836f\u7ec6\u80de\u682aG1\u671f\u7ec6\u80de\u963b\u6ede\u6bd4\u4f8b\uff0c\u6062\u590d\u62c9\u5e15\u66ff\u5c3c\u5bf9HER2+\u8010\u836f\u7ec6\u80de\u682a\u7684\u6291\u589e\u6b96\u6548\u679c\uff0c\u4ee5\u53ca\u6d53\u5ea6\u4f9d\u8d56\u5730\u6291\u5236\u8010\u836f\u7ec6\u80de\u682a\u514b\u9686\u5f62\u6210\u7684\u6570\u91cf\u3002\u6211\u4eec\u540c\u65f6\u8fd8\u4e0e\u4e34\u5e8a\u4e0a\u9009\u62e9\u6027PI3K-p110\u03b1\u6fc0\u9176\u6291\u5236\u5242Alpelisib\u8fdb\u884c\u4e86\u7597\u6548\u7684\u5bf9\u6bd4\uff0c\u53d1\u73b0\u4e0d\u8bba\u662f\u4f53\u5185\u8fd8\u662f\u4f53\u5916\uff0c\u5355\u7528\u6216\u662f\u8054\u7528\u62c9\u5e15\u66ff\u5c3c\uff0cPI3K PROTAC\u5206\u5b50\u7684\u6297\u80bf\u7624\u6d3b\u6027\u90fd\u8981\u663e\u8457\u4f18\u4e8eAlpelisib\u3002PI3K PROTAC\u5206\u5b50\u4f18\u8d8a\u7684\u6297\u80bf\u7624\u6d3b\u6027\u4e5f\u5728\u52a8\u7269\u5f02\u79cd\u79fb\u690d\u7624\u6a21\u578b\u53ca\u60a3\u8005\u6765\u6e90\u7684\u4e73\u817a\u764c\u7c7b\u5668\u5b98\u4e2d\u5f97\u5230\u9a8c\u8bc1\u3002\u8be5\u7814\u7a76\u53d1\u73b0PI3K PROTAC\u5206\u5b50\u662f\u4e00\u4e2a\u6781\u5177\u6f5c\u529b\u7684\u5148\u5bfc\u5316\u5408\u7269\uff0c\u4e0d\u8bba\u662f\u5355\u7528\u8fd8\u662f\u8054\u7528\uff0c\u90fd\u53ef\u4ee5\u6709\u6548\u514b\u670d\u4e34\u5e8a\u4e0aHER2\u9776\u5411\u6cbb\u7597\u8010\u836f\u7684\u96be\u9898\u3002\u6e05\u534e\u5927\u5b66\u9976\u71cf\u6559\u6388\u3001\u751f\u7269\u533b\u5b66\u5de5\u7a0b\u7814\u7a76\u9662\u9648\u7b56\u5b9e\u7814\u7a76\u5458\u53ca\u4e91\u5357\u7701\u80bf\u7624\u533b\u9662\u535a\u58eb\u540e\u5218\u6587\u5a67\u4e3a\u8be5\u8bba\u6587\u7684\u5171\u540c\u901a\u8baf\u4f5c\u8005\u3002<\/p>\n\n\n\n

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\u5de5\u4f5c\u6a21\u578b<\/figcaption><\/figure>\n\n\n\n

\u539f\u6587\u94fe\u63a5\uff1ahttps:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S030438352400507X<\/a><\/p>\n\n\n\n

3.\u6e05\u534e\u5927\u5b66\u57fa\u7840\u533b\u5b66\u9662\u3001\u5206\u5b50\u80bf\u7624\u5b66\u5168\u56fd\u91cd\u70b9\u5b9e\u9a8c\u5ba4\u4e3b\u8981\u6210\u5458\u90ed\u6653\u6b22\u526f\u6559\u6388\u8bfe\u9898\u7ec4\u4e8e2024\u5e747\u670813\u65e5\u5728\u300aTrends in Endocrinology & Metabolism\u300b\u6742\u5fd7\u53d1\u8868\u4e86\u9898\u4e3a \u201cGut microbial metabolites in cancer therapy\u201d\u7684\u7efc\u8ff0\u6587\u7ae0\u3002\u7cfb\u7edf\u6027\u7684\u603b\u7ed3\u4e86\u80a0\u9053\u83cc\u7fa4\u4ee3\u8c22\u4ea7\u7269\u5728\u80bf\u7624\u6cbb\u7597\u4e2d\u7684\u91cd\u8981\u4f5c\u7528\u3002\u6e05\u534e\u5927\u5b66\u57fa\u7840\u533b\u5b66\u9662\u90ed\u6653\u6b22\u526f\u6559\u6388\u548c\u5317\u4eac\u5927\u5b66\u80bf\u7624\u533b\u9662\u5f6d\u667a\u526f\u6559\u6388\u4e3a\u672c\u6587\u7684\u5171\u540c\u901a\u8baf\u4f5c\u8005\uff0c\u57fa\u7840\u533b\u5b66\u9662\u535a\u58eb\u751f\u5b8b\u78d0\u5a01\u4e3a\u7b2c\u4e00\u4f5c\u8005\u3002<\/p>\n\n\n\n

\"\"
\u8272\u6c28\u9178\u76f8\u5173\u7684\u80a0\u9053\u83cc\u7fa4\u4ee3\u8c22\u4ea7\u7269\u4e0e\u80bf\u7624\u6cbb\u7597<\/figcaption><\/figure>\n\n\n\n

\u539f\u6587\u94fe\u63a5\uff1a<\/p>\n\n\n\n

https:\/\/www.cell.com\/trends\/endocrinology-metabolism\/abstract\/S1043-2760(24)00177-2<\/a><\/p>\n\n\n\n

4.\u6e05\u534e\u5927\u5b66\u751f\u547d\u79d1\u5b66\u5b66\u9662\u3001\u5206\u5b50\u80bf\u7624\u5b66\u5168\u56fd\u91cd\u70b9\u5b9e\u9a8c\u5ba4\u4e3b\u8981\u6210\u5458\u6c5f\u9e4f\u526f\u6559\u6388\u8bfe\u9898\u7ec4\u4e8e2024\u5e747\u670826\u65e5\u5728\u300anature chemical biology\u300b\u671f\u520a\u4e0a\u53d1\u8868\u4e86\u9898\u4e3a” Metabolic determinants of germinal center B cell formation and responses”\u7684\u7814\u7a76\u8bba\u6587\u3002\u6742\u5fd7\u5f53\u671f\u9644\u6709news and views\uff0c\u5f3a\u8c03\u4e86\u53d1\u73b0\u7684\u91cd\u8981\u610f\u4e49\u3002\u5728\u672c\u9879\u5de5\u4f5c\u4e2d\uff0c\u7814\u7a76\u4eba\u5458\u63ed\u793a\u4e86\u9ad8\u6c34\u5e73\u7684\u6838\u82f7\u9178\u5408\u6210\u548c\u6c27\u5316\u8fd8\u539f\u5e73\u8861\u80fd\u529b\u662f GC B \u7ec6\u80de\u5f62\u6210\u548c\u53cd\u5e94\u6240\u5fc5\u9700\u7684\uff0c\u4ece\u800c\u63ed\u793a\u4e86\u51b3\u5b9aGC B \u7ec6\u80de\u547d\u8fd0\u7684\u4ee3\u8c22\u57fa\u7840\u548c\u5173\u952e\u751f\u5316\u56e0\u7d20\u3002<\/p>\n\n\n\n

\"\"
\u4e00\u78b3\u4ee3\u8c22\u4ecb\u5bfc\u7684\u6838\u82f7\u9178\u5408\u6210\u548c\u6297\u6c27\u5316\u80fd\u529b\u5f71\u54cdGC B \u7ec6\u80de\u5f62\u6210<\/figcaption><\/figure>\n\n\n\n

\u539f\u6587\u94fe\u63a5\uff1a<\/p>\n\n\n\n

https:\/\/doi.org\/10.1038\/s41589-024-01690-6<\/a><\/p>\n\n\n\n

8\u6708<\/strong><\/h2>\n\n\n\n

1.\u534e\u5927\u5b66\u836f\u5b66\u9662\u3001\u5206\u5b50\u80bf\u7624\u5b66\u5168\u56fd\u91cd\u70b9\u5b9e\u9a8c\u5ba4\u4e3b\u8981\u6210\u5458\u5f20\u6570\u4e00\u526f\u6559\u6388\u8bfe\u9898\u7ec4\u4e8e2024\u5e748\u67081\u65e5\u5728\u300aNucleic Acids Research\u300b\u4e0a\u53d1\u8868\u4e86\u9898\u4e3a”CRISPR perfect adaptation for robust control of cellular immune and apoptotic responses”\u7684\u7814\u7a76\u6587\u7ae0\u3002\u5728\u8fd9\u9879\u7814\u7a76\u4e2d\uff0c\u4f5c\u8005\u6784\u5efa\u5e76\u9a8c\u8bc1\u4e86\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u4e2d\u7684RPA\u7cfb\u7edf\uff0c\u5e76\u5e94\u7528\u4e8e\u514d\u75ab\u7ec6\u80deNF-\u03baB\u901a\u8def\u548c\u7ec6\u80de\u51cb\u4ea1\u901a\u8def\uff0c\u4e3a\u5185\u6e90\u57fa\u56e0\u8868\u8fbe\u5f02\u5e38\u7684\u6cbb\u7597\u63d0\u4f9b\u65b0\u601d\u8def\u3002<\/p>\n\n\n\n

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RPA\u7cfb\u7edf\u7406\u8bba\u6a21\u578b<\/figcaption><\/figure>\n\n\n\n

\u539f\u6587\u94fe\u63a5\uff1a<\/p>\n\n\n\n

https:\/\/academic.oup.com\/nar\/advance-article\/doi\/10.1093\/nar\/gkae665\/7725472?login=true<\/a><\/p>\n\n\n\n

2.\u6e05\u534e\u5927\u5b66\u57fa\u7840\u533b\u5b66\u9662\u3001\u5206\u5b50\u80bf\u7624\u5b66\u5168\u56fd\u91cd\u70b9\u5b9e\u9a8c\u5ba4\u4e3b\u8981\u6210\u5458\u90d1\u64bc\u7403\u526f\u6559\u6388\u8bfe\u9898\u7ec4\u548c\u9752\u5c9b\u5927\u5b66\u9644\u5c5e\u533b\u9662\u5cb3\u658c\u56e2\u961f\u5408\u4f5c\u4e8e2024\u5e748\u670815\u65e5\u5728\u300aNature Cell Biology\u300b\u671f\u520a\u4e0a\u53d1\u8868\u4e86\u9898\u4e3a”Lymphotoxin-\u03b2 promotes breast cancer bone metastasis colonization and osteolytic outgrowth”\u7684\u7814\u7a76\u8bba\u6587\u3002\u6742\u5fd7\u7f16\u8f91\u90e8\u4e5f\u5c06\u4e3a\u8be5\u8bba\u6587\u914d\u53d1Research briefing\u7684\u4eae\u70b9\u6587\u7ae0\u63a8\u8350\u3002\u5728\u672c\u9879\u7814\u7a76\u4e2d\uff0c\u7814\u7a76\u4eba\u5458\u901a\u8fc7\u5bf9\u9aa8\u8f6c\u79fb\u65e9\u671f\u5b9a\u690d\u80bf\u7624\u7ec6\u80de\u7684\u5355\u7ec6\u80deRNA-Seq\u5206\u6790\u53ca\u4f53\u5185\u7b5b\u9009\uff0c\u9274\u5b9a\u4e86\u6dcb\u5df4\u6bd2\u7d20-\u03b2\uff08Lymphotoxin-\u03b2\uff0cLT\u03b2\uff09\u5728\u4fc3\u8fdb\u4e73\u817a\u764c\u9aa8\u8f6c\u79fb\u5b9a\u690d\u548c\u8fdb\u5c55\u7684\u5173\u952e\u4f5c\u7528\u3002<\/p>\n\n\n\n

\"\"
\u6dcb\u5df4\u6bd2\u7d20-\u03b2\u534f\u52a9\u764c\u75c7\u9aa8\u8f6c\u79fb\u7684\u5b9a\u690d\u548c\u6eb6\u9aa8\u6027\u751f\u957f<\/figcaption><\/figure>\n\n\n\n

\u539f\u6587\u94fe\u63a5\uff1a<\/p>\n\n\n\n

https:\/\/www.nature.com\/articles\/s41556-024-01478-9<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

7\u6708 1.\u6e05\u534e\u5927\u5b66\u57fa\u7840\u533b\u5b66\u9662\u3001\u5206\u5b50\u80bf\u7624\u5b66\u5168\u56fd\u91cd\u70b9\u5b9e\u9a8c\u5ba4\u4e3b\u8981\u6210 […]<\/p>\n","protected":false},"author":1,"featured_media":9757,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[23],"tags":[],"class_list":["post-9753","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-keyan"],"_links":{"self":[{"href":"https:\/\/sklmo.tsinghua.edu.cn\/index.php?rest_route=\/wp\/v2\/posts\/9753","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sklmo.tsinghua.edu.cn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sklmo.tsinghua.edu.cn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sklmo.tsinghua.edu.cn\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sklmo.tsinghua.edu.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9753"}],"version-history":[{"count":1,"href":"https:\/\/sklmo.tsinghua.edu.cn\/index.php?rest_route=\/wp\/v2\/posts\/9753\/revisions"}],"predecessor-version":[{"id":9761,"href":"https:\/\/sklmo.tsinghua.edu.cn\/index.php?rest_route=\/wp\/v2\/posts\/9753\/revisions\/9761"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sklmo.tsinghua.edu.cn\/index.php?rest_route=\/wp\/v2\/media\/9757"}],"wp:attachment":[{"href":"https:\/\/sklmo.tsinghua.edu.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9753"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sklmo.tsinghua.edu.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9753"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sklmo.tsinghua.edu.cn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9753"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}